California Agriculture Online
California Agriculture Home  >   Volume 36   >   Number 8  >   Viewing Expanded Abstract

peer-reviewed research article

Protoplast regeneration

authors

Dean E. Engler, U.C., Davis.
Raymond G. Grogan, U.C., Davis.

publication information

California Agriculture 36(8):18-19. DOI: 10.3733/ca.v036n08p18. August 1982.

abstract

Not available – first paragraph follows:

Plant cells without walls (protoplasts) can be isolated from leaves by a process of enzy-matically digesting away the middle lamellae between cells and the cell walls. Tremendously large numbers of protoplasts can be isolated from a single leaf; yields are typically two to four million protoplasts per gram of leaf tissue. Development of techniques and procedures causing isolated protoplasts to reform their walls, proliferate, and regenerate into whole plants is essential for the utilization of the new genetic technology.

author affiliations

Dean E. Engler, Research Assistant, U.C., Davis. Raymond G. Grogan, Professor, Plant Pathology, U.C., Davis.


  • Search Article Text 1946 to Present
Advanced Search